WebThe PhoH protein was overproduced by the T7 promoter system, identified as a protein of about 39 kDa, and purified. The amino-terminal amino acid sequence of the PhoH protein agreed with the one deduced from the DNA sequence. We demonstrated that PhoH has an ATP-binding activity by a photoaffinity labeling experiment.

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WebOct 15, 2011 · Comparison of phosphoprotein and phosphopeptide enrichment. Protein extraction represents the first step for both phosphoprotein and phosphopeptide enrichment (Fig. 1).The removal of nucleic acids and other interfering contaminants is especially important for plant phosphoproteomic experiments where removal of cell-wall … WebA photoproximity labeling approach is described that allows capturing of protein interactomes using brief exposure to visible light. The technology—Light-induced … how to make jpeg smaller size

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WebMar 31, 2024 · Likewise, we found that the expressions of NYMV nucleoprotein (N) and phosphoprotein (P) alone induce the formation of condensates and puncta in the nucleus, respectively, even though their morphologies are somewhat different from the IBs observed in the actual NYMV-infected cells. In addition, IB-like structures can be reconstructed by … WebPhosphoprotein P0 is a highly conserved ribosomal protein that forms the central scaffold of the large ribosomal subunit’s “stalk complex”, which is necessary for recruiting protein elongation factors to the ribosome. Evidence in the literature suggests that P0 may be involved in diseases such as malaria and systemic lupus erythematosus. WebJan 29, 2024 · The phoH gene was detected in various morphological types of phages (including siphophages, myophages and podophages) and in a wide host range (including autotrophic and heterotrophic bacteria)... how to make jpeg to gif