Phic31 attp
WebMar 4, 2016 · In Drosophila melanogaster, the phiC31 attP site was first placed into the genome with a randomly integrating P element. The attP site was then targeted at high efficiency and specificity by an incoming plasmid bearing the attB site, utilizing co-injected mRNA encoding phiC31 integrase [ 7 ]. WebExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism Depositor Norbert Perrimon
Phic31 attp
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WebFeb 4, 2024 · Here, we adapted the phiC31 integrase system for C. elegans. We generated an integrated phiC31 integrase expressing strain flanked by attP sites that serves as a … WebphiC31 integrase: Chromosome 4 (102F4), vasa promoter, marked with GFP and RFP Chromosome 2 attP Landing Site vas-int; attP40 (Stock 13-20) Genotype: y w M (eGFP, vas-int, dmRFP)ZH-2A; P {CaryP}attP40 attP site: 2 nd chromosome (25C6), marked with yellow+ phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP
WebJul 21, 2006 · Both Bxb1 and phiC31 integrases mediate integrative recombination without a requirement for divalent cations, cofactors or DNA supercoiling, making them ideally suited for targeted gene delivery... WebMay 3, 2007 · The minimal sites for ϕC31 integrase have been defined as a 39 bp attP site and a 34 bp attB site ( 10 ). Under in vitro conditions ϕC31 integrase converts ∼80% of attB and attP to products in the absence of accessory proteins and there are no restrictions on the topology of the substrates ( 16, 27, 28 ).
WebFeb 28, 2014 · Abstract. PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are … WebEnter the email address you signed up with and we'll email you a reset link.
WebPhiC31 integrase, a site-specific large serine recombinase, is a useful tool for genome engineering in a variety of eukaryotic species and cell types. PhiC31 integrase performs efficient recombination between its attB site and either its own placed attP site or a partially mismatched genomic pseudo attP site.
WebSep 14, 2012 · The target sequence involves a reporter gene encoding green fluorescent protein (GFP), which is flanked by the attB and attP recognition sites for the phiC31 integrase. This sequence disruptively separates a gusA coding sequence from an upstream rice actin promoter. We succeeded in producing site-specific recombination events in the … the side effects of adderallWebFeb 15, 2024 · The attP site (5'-GTGCCCCAACTGGGGTAACCTttGAGTTCTCTCAGTTGGGGG-3') is one of the two target sites of the phiC31:int integrase. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core flanked by imperfect inverted repeats. my time is wrong on my pcWebFeb 15, 2024 · The attB site (5'-TGCGGGTGCCAGGGCGTGCCCttGGGCTCCCCGGGCGCGTACTCC-3') is one of the two target sites of the phiC31:int integrase. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core … my time is tightWebMar 21, 2012 · The Bxb1 recombinase is a 500 amino acid protein that binds minimal recognition sites attP and attB that are 39 bp and 34 bp, respectively and enzymatically executes uni-directional site-specific recombination [ 9 ]. the side door restaurant scottsdaleWebPhiC31 Integrase Expression Plasmid Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step … the side effects filmWebJun 17, 2010 · Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish. the side effects of cymbaltaWebthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, … the side effects of birth control pills