Web21 okt. 2024 · Then, these blood and stool samples were used to extract genomic DNA using the MiniBEST Universal Genomic DNA Extraction Kit Ver.5.0 (TaKaRa, China) and the TIANamp Stool DNA Kit (TIANGEN, China), respectively. 2 μL of genomic DNA extracted from spiked blood and stool samples were then used as templates for RAA … WebAn optimized DNA extraction kit for plasmids using a modified alkaline lysis method has a number of advantages including: Rapid purification of high-quality DNA DNA ready for PCR, sequencing, cloning, and other …
Microbial DNA Extraction Bacterial DNA Isolation Kit QIAGEN
WebPromega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. DNA … Web5 okt. 2006 · Genomic DNA extraction of E. coli was performed with MiniBEST bacterial genomic DNA extraction kit. Seven simultaneous PCRs were performed to amplify each DNA fragment. For the POEP method (Fig. 1 ), the first PCRs were done on a PE thermocycler 9600 for 10 cycles with 1 U pyrobest Taq polymerase, 30 pmol of each … francis s key
核酸、蛋白质提取及纯化制品引言 - Takara
WebThis kit is designed for the rapid spin column preparation of genomic DNA from 2 x 10 9 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both … Web21 jun. 2024 · DNA extraction was performed using the TaKaRa MiniBEST Bacterial Genomic DNA Extraction Kit ver. 3.0 (Takara Bio, Kusatsu, Japan), according to the manufacturer’s protocol. The 16S rRNA gene sequences of the isolates were amplified using the universal bacterial primer sets 27F and 805R as well as 518F and 1492R, and … WebMiniBEST Bacterial Genomic DNA Extraction kits (Takara, Dalian, China), and the phenol-chloroform and boiling lysis methods. The concentration of the genomic DNA was measured using a spectrophotometer. The purity of the genomic DNA was evaluated by PCR amplification of 16S rDNA followed by determining the cloning efficiency of blank tea towels bulk